| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Martin, Catherine L (10)
|
MAHAC |
41.35 |
40.11 |
-1.24 |
| 2 |
Benka-Davies, Zoe M (10)
|
MACA |
46.49 |
44.70 |
-1.79 |
| 3 |
Blacker, Elaine A (10)
|
MAHAC |
46.54 |
46.88 |
0.34 |
| 4 |
Lyn-Sue, Rachel E (10)
|
MAHAC |
|
47.17 |
- |
| 5 |
Serfass, Madeline G (9)
|
MAHAC |
49.39 |
50.39 |
1.00 |
| 6 |
Lee, Madison M (10)
|
MACA |
53.87 |
50.62 |
-3.25 |
| 7 |
Zoellner, Aubrey G (10)
|
MACA |
51.10 |
51.03 |
-0.07 |
| 8 |
Kulasinsky, Adrienne J (9)
|
MAHAC |
51.50 |
51.19 |
-0.31 |
| 9 |
Leonard, Allison J (9)
|
MAHAC |
1:05.55 |
52.61 |
-12.94 |
| 10 |
Zaprazny, Julia (10)
|
MAHAC |
50.57 |
52.71 |
2.14 |
| 11 |
D'Souza, Rachel N (10)
|
MACA |
55.97 |
52.77 |
-3.20 |
| 12 |
Woronko, Julia M (8)
|
MAHAC |
53.13 |
54.53 |
1.40 |
| 13 |
Khoshneissan, Ruvarashe H (9)
|
MACA |
|
59.41 |
- |
| 14 |
Thrush, Hannah M (9)
|
MACA |
1:01.96 |
1:00.09 |
-1.87 |
| 15 |
Megonnell, Alexis R (9)
|
MACA |
|
1:02.04 |
- |